superscript iii reverse transcriptase (Fisher Scientific)

About

News

Press Release

Team

Advisors

Partners

Contact

Bioz Stars

Bioz vStars

Buy from Supplier

Structured Review

Fisher Scientific superscript iii reverse transcriptase
Superscript Iii Reverse Transcriptase, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/superscript+iii+reverse+transcriptase/m+mlv+reverse+transcriptase/pmc13157194-36-0-5
Average 86 stars, based on 1 article reviews

superscript iii reverse transcriptase - by Bioz Stars, 2026-09

86/100 stars

Images

Reverse Transcription:

Article Title: Expansion of myeloid suppressor cells and suppression of Lassa virus-specific T cells during fatal Lassa fever.
Article Snippet: DNA was digested using a DNAse and total RNA was extracted using an RNeasy Mini kit (Qiagen), eluted in RNAse-free water, and stored at -80°C before processing. .. Nucleic acid was denatured and exposed to SuperScript III reverse transcriptase (FisherScientific) and the cDNA was mixed with primers and probes specific for genes of interest and DNA polymerase AmpliTaq Gold (FisherScientific) for amplification. ..

Article Title: Fisetin ameliorates vascular smooth muscle cell calcification via DUSP1-dependent p38 MAPK inhibition
Article Snippet: .. Total RNA was isolated by using Trizol Reagent and reverse transcription was performed by using oligo(dT) 12-18 primers and SuperScript III Reverse Transcriptase (all from Fisher Scientific). .. RT-PCR was performed with iQ Sybr Green Supermix (Bio-Rad Laboratories) and the primers listed below (Fisher Scientific) [ – ].

Article Title: Seamless, modular binary vectors for plant cell and molecular biology
Article Snippet: .. RNA was extracted from A. thaliana Col‐0 seedlings using the Spectrum Plant Total RNA (Sigma‐Aldrich) kit with on‐column DNase I digestion (New England Biolabs). cDNA was synthesized from this RNA using random hexamers and SuperScript III reverse transcriptase (Fisher Scientific). .. The H2B coding sequence was amplified with primers 5′‐caacaccagaatctggatccATGGCGAAGGCAGATAAGAAACC‐3′ and 5′‐ggagtttcagagccggatccAGAACTCGTAAACTTCGTAACCGC‐3′, the Reptin coding sequence was amplified with primers 5′‐caacaccagaatctggatccATGGCGGAACTAAAGCTATCAG‐3′ and 5′‐ggagtttcagagccggatccGATCTGCATAGCATCTTGTTCGTC‐3′, and the Pontin coding sequence was amplified with primers 5′‐caacaccagaatctggatccATGGAGAAAGTAAAGATTGAAGAAATTCA‐3′ and 5′‐ggagtttcagagccggatccTGAGATGTATTTTTCTTGTTGCTCATG‐3′ and using Phusion DNA polymerase (New England Biolabs) and purified using a Monarch Nucleic Acid Purification kit (New England Biolabs). pAVEC1::C‐mEGFP and pAVEC1::C‐mTurboID were digested with BamHI‐HF (New England Biolabs), purified using a Monarch Nucleic Acid Purification kit (New England Biolabs), and assembled with the amplified coding sequences using the one‐step T4 DNA polymerase assembly method (Islam et al ., ; Jeong et al ., ).

Article Title: Sphingosine kinase 1 inhibition aggravates vascular smooth muscle cell calcification.
Article Snippet: .. Total RNA was isolated by using Trizol Reagent (Fisher Scientific), and reverse transcription was performed by using SuperScript III Reverse Transcriptase and oligo(dT)12–18 primers (Fisher Scientific). .. RT-PCR was performed in duplicate with iQ Sybr Green Supermix (Bio-Rad Laboratories) and the following primers (Fisher Scientific) [69, 72]: Human primers: ALPL fw: GGG ACT GGT ACT CAG ACA ACG ALPL rev: GTA GGC GAT GTC CTT ACA GCC CBFA1 fw: GCC TTC CAC TCT CAG TAA GAAGA CBFA1 rev: GCC TGG GGT CTG AAA AAG GG GAPDH fw: GAG TCA ACG GAT TTG GTC GT GAPDH rev: GAC AAG CTT CCC GTT CTC AG SPHK1 fw: GGC TGC TGT CAC CCA TGA A SPHK1 rev: TCA CTC TCT AGG TCC ACA TCAG Mouse primers: Alpl fw: TTG TGC CAG AGA AAG AGA GAGA Alpl rev: GTT TCA GGG CAT TTT TCA AGGT Cbfa1 fw: AGA GTC AGA TTA CAG ATC CCAGG Cbfa1 rev: AGG AGG GGT AAG ACT GGT CATA Gapdh fw: AGG TCG GTG TGA ACG GAT TTG Gapdh rev: TGT AGA CCA TGT AGT TGA GGTCA Relative mRNA fold changes were calculated by the 2−ΔΔCt method using GAPDH as internal reference.

Article Title: Characterization and comparison of two NPC cell lines, C17 and C666-1, as models for studying the pathogenesis of nasopharyngeal carcinoma.
Article Snippet: .. SuperScript III Reverse Transcriptase was obtained from InvitrogenThermo Fisher Scientific (Darmstadt, Germany; #18080044). .. The RNeasy Mini Kit was purchased from Qiagen (Hilden, Germany; #74104).

Article Title: CFIm25-dependent alternative polyadenylation in AKT2 mRNA programs macrophage polarization
Article Snippet: Anchored Oligo dT (22) primer , IDT , Cat# 51-01-15-09. .. Superscript III Reverse Transcriptase , Fisher Scientific , Cat# 18-080-093. .. Trypan Blue staining solution (0.4%) , Thermo Fisher Scientific , Cat# 15250061.

Article Title: Fisetin ameliorates vascular smooth muscle cell calcification via DUSP1-dependent p38 MAPK inhibition.
Article Snippet: .. Total RNA was isolated by using Trizol Reagent and reverse transcription was performed by using oligo(dT)12-18 primers and SuperScript III Reverse Transcriptase (all from Fisher Scientific). .. RT-PCR was performed with iQ Sybr Green Supermix (Bio-Rad Laboratories) and the primers listed below (Fisher Scientific) [56–58].

Amplification:

Isolation:

Synthesized:

other:

Article Title: Immunostimulation of Asian elephant ( Elephas maximus ) blood cells by parapoxvirus ovis and CpG motif-containing bacterial plasmid DNA upregulates innate immune gene expression
Article Snippet: A mixture of the following reagents was then added: 200 units of InvitrogenTM SuperscriptTM III Reverse Transcriptase (Fisher Scientific), 40 units of recombinant RNasin ® ribonuclease inhibitor (Promega UK Ltd.), 1 μL of kit-supplied 0.1 M dithiothreitol (DTT) and 4 μL of kit-supplied 5x First-Strand Buffer.

Review